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By P. Davide Cozzoli, Liberato Manna (auth.), Warren C. W. Chan Ph.D. (eds.)

ISBN-10: 0387767126

ISBN-13: 9780387767123

In this edited ebook, we spotlight the relevant avid gamers within the Bionanotechnology box, that are the nanostructures and biomolecules. The publication starts off by means of describing how nanostructures are synthesized and through describing the wide range of nanostructures to be had for organic study and purposes. additionally proven are the recommendations used to synthesize a wide selection of organic molecules. subsequent, there's a specialize in the meeting of nanostructures with organic molecules, that can result in the layout of multi-functional nanosystems. within the following chapters, examples of the original homes of nanostructures are supplied besides the present purposes of those nanostructures in biology and medication. a few purposes comprise using gold nanoparticles in diagnostic functions, quantum dots and silica nanoparticles for imaging, and liposomes for drug supply. within the ultimate chapters of the ebook, the toxicity of nanostructures are defined. This ebook offers huge examples of present advancements in Bionanotechnology examine and will be a great advent to the field.

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65. Mokari T, Rothenberg E, Popov I et al. Science 2004; 304(5678):1787. 66. Kudera S, Carbone L, Casula MF et al. Nano Lett 2005; 5(3):445. 67. Mokari T, Sztrum CG, Salant A et al. Nat Mater 2005; 4:855. 68. Milliron DJ, Hughes SM, Cui Y et al. Nature 2004; 430(6996):190. 69. Shevchenko EV, Talapin DV, Rogach AL et al. J Am Chem Soc 2002; 124(38):11480. 70. Chen CC, Chao CY, Lang ZH. Chem Mater 2000; 12:1516. 71. Jana NR, Gearheart L, Murphy CJ. J Phys Chem B 2001; 105(19):4065. 72. Trentler TJ, Hickman KM, Goel SC et al.

55 Current Approaches for Engineering Proteins with Diverse Biological Properties 25 Figure 5. Staggered Extension Process (StEP). There are three double-stranded homologous parental genes that are distinguished by their shade and used as PCR templates. In the first PCR cycle, the primer anneals to the single-stranded parental template after melting. Following annealing, the extension time of the PCR reaction is short enough to allow only partial extension of the primer on the parental gene. The same process is repeated in subsequent cycles until the extended primer reaches full length as the parental genes.

Visualizing the mechanical activation of Src. Nature 2005; 434(7036):1040-1045. 82. Chiang JJ, Li I, Truong K. Creation of circularly permutated yellow fluorescent proteins using fluorescence screening and a tandem fusion template. Biotechnol Lett 2006; 28(7):471-475. 83. Nakamura T, Iwakura M. Circular permutation analysis as a method for distinction of functional elements in the M20 loop of Escherichia coli dihydrofolate reductase. J Biol Chem 1999; 274(27):19041-19047. 84. Iwakura M, Nakamura T, Yamane C et al.

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Bio-Applications of Nanoparticles by P. Davide Cozzoli, Liberato Manna (auth.), Warren C. W. Chan Ph.D. (eds.)

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