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Advances in Low-Temperature Biology, Volume 3 - download pdf or read online

By Peter L. Steponkus

ISBN-10: 0080552285

ISBN-13: 9780080552286

ISBN-10: 0762301600

ISBN-13: 9780762301607

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Extra info for Advances in Low-Temperature Biology, Volume 3

Sample text

Moreover, Kondo et al. (1974) tried to improve their method of prolonged cardiac arrest with profound hypothermia by perfusing the vital organs such as brain, heart, and lungs in dogs with a so-called "intracellular-type" solution. Using Collins' kidney preservation solution containing high concentrations of potassium, magnesium, phosphate and glucose, they reported the successful resuscitation of a group of dogs after 21/2 hours of circulatory arrest at 1TC. However, several animals died acutely showing evidence of respiratory distress and autopsy revealed pulmonary congestion, edema and alveolar hemorrhage.

Ultraprofound hypothermia with blood substitution (UHBS) is the approach we have explored by paying particular attention to the nature of the solutions used to substitute for the blood. Before describing this approach in detail, it is informative to review previous experimental attempts to employ extreme hemodilution and profound hypothermia as a potential strategy for ameliorating ischemic problems in the quest for techniques to extend the safe interval of arrest for bloodless surgery. HISTORICAL BASIS FOR ULTRAPROFOUND HYPOTHERMIA AND BLOOD SUBSTITUTION The concept of total blood removal in conjunction with hypothermia is not new but has not been explored extensively (Negovskii, 1959; Boerema, 1960; Dogliotti, 17 18 MJ.

Conceptually, it has been argued that a hyperkalemic solution would be beneficial by inhibiting the passive exchange of monovalent cations across cell membranes during hypothermic exposure when active membrane pumps are suppressed. , 1985; 1989)). With reference again to the earlier attempts by Kondo et al.

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Advances in Low-Temperature Biology, Volume 3 by Peter L. Steponkus

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